|
Status |
Public on Mar 01, 2010 |
Title |
Peripheral Whole Blood 105243 |
Sample type |
genomic |
|
|
Source name |
genomic DNA from whole blood 105243
|
Organism |
Homo sapiens |
Characteristics |
sample type: bi-sulphite converted genomic whole blood DNA from Case agegroupatsampledraw: 70 to 75 ageatrecruitment: 74 ageatdiagnosis: 74 histology: Serous stage: IIIb grade: Grade 3 pre-treatment sample: No post-treatment sample: Yes ca125: 23.37 batch: 9 beadchip_well: 4447820162_E bs conversion c1: Grn 8636 bs conversion c2: Grn 8682
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted at Tepnel (www.tepnel.com) using a chloroform extraction method and 800ng DNA from each sample was bisulphite converted using the EZ DNA Methylation Kit D5008 (Zymo Research, Orange, CA, USA)
|
Label |
as per Illumina Infinium 27k methylation assay protocol
|
Label protocol |
A single base extension was performed using labelled DNP- and biotin labelled dNTPs. See the Illumina Infinium 27k methylation assay protocol.
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|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchip
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended scanner setting
|
Description |
whole blood from cases are from postmenopausal women with ovarian cancer, drawn either before or after treatment
|
Data processing |
BeadStudio software v3.2
|
|
|
Submission date |
Dec 31, 2009 |
Last update date |
Mar 01, 2010 |
Contact name |
Andrew E Teschendorff |
E-mail(s) |
a.teschendorff@ucl.ac.uk
|
Phone |
+44 02076790727
|
Organization name |
University College London
|
Department |
UCL Cancer Institute/Institute for Women's Health
|
Street address |
72 Huntley Street
|
City |
London |
ZIP/Postal code |
WC1E 6BT |
Country |
United Kingdom |
|
|
Platform ID |
GPL8490 |
Series (1) |
GSE19711 |
Genome wide DNA methylation profiling of United Kingdom Ovarian Cancer Population Study (UKOPS) |
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