|
| Status |
Public on Dec 30, 2009 |
| Title |
IP-Gb |
| Sample type |
RNA |
| |
|
| Source name |
shGFP inf. Ink/Arf/PTEN ko astroc.
|
| Organism |
Mus musculus |
| Characteristics |
tumor tissue source: Cell line: 1st deg. culture
tumor organ site: Brain
pathological diagonosis: Normal
tumor stage: Non-Tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Micro-to-Midi Total RNA Purification System (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
|
| Description |
Ink4a/Arf-/- Pten-/- primary mouse astrocytes
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 28, 2009 |
| Last update date |
Dec 29, 2009 |
| Contact name |
Hailei Zhang |
| E-mail(s) |
hailei_zhang@dfci.harvard.edu
|
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
44 Binney Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE19687 |
shGFP- and shQk-transduced Ink4a/Arf-/- Pten-/- primary mouse astrocytes |
| GSE19693 |
STAR RNA-binding protein, Quaking, suppresses cancer via regulation of microRNA |
|
