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Status |
Public on Aug 19, 2010 |
Title |
Brain (Inferior Olivary Nucleus)_Control_case5 |
Sample type |
RNA |
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Source name |
Brain (Inferior Olivary Nucleus)_control subject_#5
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Organism |
Homo sapiens |
Characteristics |
sample: #15 subject: Control case #5 brain: T-2017 age: 74 sex: Male region: Inferior olivary nucleus
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Biomaterial provider |
New York Brain Bank
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Invitrogen's TRIzol reagent and purified with RNeasy mini columns (Qiagen), following the manufactures protocols.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (GeneChip Expression Analysis Technical Manual, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 expression array.
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Scan protocol |
Affymetrix Human Genome U133A 2.0 array was scanned on Affymetrix’s GeneChip Scanner 3000 7G and reviewed for quality control.
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Description |
Gene expression data from the inferior olivary nucleus in a control subject.
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Data processing |
HG-U133A 2.0 microarray was analyzed with Affymetrix Microarray Suite v5.0 and GeneSpring v5.0.3 (Silicon Genetics) software.
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Submission date |
Dec 20, 2009 |
Last update date |
Jun 09, 2010 |
Contact name |
scott small |
Organization name |
Columbia University
|
Street address |
630 W 168th st
|
City |
new york |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
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Platform ID |
GPL571 |
Series (1) |
GSE19587 |
Imaging-guided microarray: Identifies molecular markers in the pathogenesis of Parkinson’s disease |
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