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Sample GSM488132 Query DataSets for GSM488132
Status Public on Aug 19, 2010
Title Brain (Inferior Olivary Nucleus)_Control_case5
Sample type RNA
 
Source name Brain (Inferior Olivary Nucleus)_control subject_#5
Organism Homo sapiens
Characteristics sample: #15
subject: Control case #5
brain: T-2017
age: 74
sex: Male
region: Inferior olivary nucleus
Biomaterial provider New York Brain Bank
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Invitrogen's TRIzol reagent and purified with RNeasy mini columns (Qiagen), following the manufactures protocols.


Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (GeneChip Expression Analysis Technical Manual, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 expression array.
Scan protocol Affymetrix Human Genome U133A 2.0 array was scanned on Affymetrix’s GeneChip Scanner 3000 7G and reviewed for quality control.
Description Gene expression data from the inferior olivary nucleus in a control subject.
Data processing HG-U133A 2.0 microarray was analyzed with Affymetrix Microarray Suite v5.0 and GeneSpring v5.0.3 (Silicon Genetics) software.
 
Submission date Dec 20, 2009
Last update date Jun 09, 2010
Contact name scott small
Organization name Columbia University
Street address 630 W 168th st
City new york
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL571
Series (1)
GSE19587 Imaging-guided microarray: Identifies molecular markers in the pathogenesis of Parkinson’s disease

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 169.193 P 0.000753643
AFFX-BioB-M_at 278.967 P 0.000258358
AFFX-BioB-3_at 128.435 P 0.0113844
AFFX-BioC-5_at 475.986 P 4.42873e-05
AFFX-BioC-3_at 486.37 P 4.42873e-05
AFFX-BioDn-5_at 1171.11 P 4.42873e-05
AFFX-BioDn-3_at 2227.51 P 4.42873e-05
AFFX-CreX-5_at 3151.03 P 4.42873e-05
AFFX-CreX-3_at 3379.72 P 4.42873e-05
AFFX-DapX-5_at 3.23916 A 0.834139
AFFX-DapX-M_at 32.4342 A 0.227636
AFFX-DapX-3_at 49.0607 P 0.0429619
AFFX-LysX-5_at 17.2667 A 0.440646
AFFX-LysX-M_at 19.0761 A 0.51489
AFFX-LysX-3_at 22.6567 M 0.0542134
AFFX-PheX-5_at 1.38727 A 0.995516
AFFX-PheX-M_at 2.91543 A 0.979978
AFFX-PheX-3_at 12.9483 A 0.712257
AFFX-ThrX-5_at 1.79842 A 0.941556
AFFX-ThrX-M_at 3.60618 A 0.932322

Total number of rows: 22277

Table truncated, full table size 674 Kbytes.




Supplementary file Size Download File type/resource
GSM488132.CEL.gz 2.3 Mb (ftp)(http) CEL
GSM488132.CHP.gz 119.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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