|
| Status |
Public on Oct 10, 2020 |
| Title |
04-NAP1KD2 |
| Sample type |
SRA |
| |
|
| Source name |
S2_NAP1KD
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2
genotype/variation: NAP-1 knockdown
|
| Growth protocol |
S2 cells were grown in Schneider’s Drosophila media (Gibco) with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
S2 cells were collected.Total RNA was isolated using ISOGEN II reagent (NIPPON GENE), according to the manufacturer’s protocol.
RNA-seq libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
04-NAP1KD2_S4_L001
|
| Data processing |
Basecalls performed using Illumina MiSeq
RNA-seq reads were aligned to the Drosophila dm3 genome assembly using Illumina MiSeq
Genome_build: Drosophila dm3
Supplementary_files_format_and_content: RPKM
|
| |
|
| Submission date |
Oct 09, 2020 |
| Last update date |
Oct 10, 2020 |
| Contact name |
Mitsuhiro Yoneda |
| E-mail(s) |
yonedam@nagasaki-u.ac.jp
|
| Phone |
81-95-819-7038
|
| Organization name |
Nagasaki University
|
| Street address |
1-12-4 Sakamoto
|
| City |
Nagasaki |
| ZIP/Postal code |
8528523 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16479 |
| Series (1) |
| GSE159294 |
Nucleosome assembly protein-1 (NAP-1) is regulator of histone H1 acetylation |
|
| Relations |
| BioSample |
SAMN16403297 |
| SRA |
SRX9269759 |
