|
| Status |
Public on Feb 23, 2023 |
| Title |
LNCAP CONTROL #2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCAP CELLS CONTROL
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PROSTATE TUMOR CELL LINE
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNEAsy mini kit Qiagen
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human gene 1.0 ST. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Genechip Scanner 3000 7G
|
| Description |
GENE EXPRESSION FROM LNCAP CELLS
|
| Data processing |
The data were analyzed with Expression console (Affymetrix) (RMA) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 28, 2020 |
| Last update date |
Feb 23, 2023 |
| Contact name |
Pedro Nicolau Neto |
| E-mail(s) |
pedronicolau.n@gmail.com
|
| Organization name |
INCA
|
| Department |
PCM
|
| Street address |
37 Andre Cavalcanti Street
|
| City |
Rio de Janeiro |
| State/province |
RJ |
| ZIP/Postal code |
20231-050 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE158643 |
EXPRESSION DATA OF PROSTATE CELL LINES DU145 AND LNCAP AND HUMAN ASTROCYTES CO-CULTURED |
|
