|
| Status |
Public on Dec 05, 2009 |
| Title |
Weise_1-2_Col-WT_Rep2_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
Columbia0
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: Col0
genotype: wild type
developmental stage: 3.70
tissue: leaves
|
| Growth protocol |
Plants were sewn in flats of modified commercial soil and grown in a growth chamber with a 12 hour photoperiod, quantum flux of 100 micromol m-2s-1, relative humidity was at least 75%, and a constant temperature of 20 degrees Celsius
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Leaf material was powered using a mortar and pestle and RNA was extracted using a Purescript RNA isolation kit (Gentra Systems, MN, USA)according to kit directions. Samples were then treated with DNaseI (Invitrogen, CA, USA) according to manufactures directions
|
| Label |
biotin
|
| Label protocol |
Labelled using protocol described in manual "Affymetrix GeneChip Expression Analysis Technical Manual", One-Cycle target labelling
|
| |
|
| Hybridization protocol |
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
|
| Scan protocol |
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.
|
| Description |
Growth chamber Levington F2 (Fine peat/with intercept an insecticide) with extra grit 4mm at 40%. 75% 20oC. Three leaves from 8 different plants of each genotype were harvested using a green safe lamp 0.5 hours before the lights came on in the morning and immediately frozen in LN2.
|
| Data processing |
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
|
| |
|
| Submission date |
Dec 02, 2009 |
| Last update date |
Dec 04, 2009 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
affy@arabidopsis.info
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE19260 |
Transcript inhibition from limited starch degradation |
|
