|
| Status |
Public on Sep 08, 2020 |
| Title |
ink128.1 |
| Sample type |
SRA |
| |
|
| Source name |
rOvary tissue
|
| Organism |
Mus musculus |
| Characteristics |
treatment: INK128
|
| Treatment protocol |
For mice，the INK128 was fed at a final concentration of 0.75uM/g in drinking water（based on the weight of mice, weigh the mice once a week）.
|
| Growth protocol |
The rOvaries were obtained from mice after transplated one month
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
The RNA of rOvaries were extracted by Rneasy Plus Mini Kit(Cat. No. 74134)
Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
RNA-seq raw reads with low-quality bases, adapters were trimmed by Trimmomatic to obtain clean reads
clean reads were aligned to the UCSC mouse mm10 reference genome using the Hisat2 with default settings
Read counts of each gene annotated in RefGene were calculated by featureCounts
Genome_build: mm10
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Sep 02, 2020 |
| Last update date |
Sep 08, 2020 |
| Contact name |
Heng Dai |
| E-mail(s) |
1120180417@mail.nankai.edu.cn
|
| Phone |
15201113160
|
| Organization name |
NanKai University
|
| Street address |
NanKai District NanKai University
|
| City |
TianJin |
| State/province |
TianJin |
| ZIP/Postal code |
300071 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE157325 |
mTOR inhibition by INK128 extends ovarian functions in aging and premature aging mice following germline stem cell transplantation |
|
| Relations |
| BioSample |
SAMN15964849 |
| SRA |
SRX9057376 |
