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| Status |
Public on Sep 29, 2020 |
| Title |
WT_Retina |
| Sample type |
SRA |
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| Source name |
Retina
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
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| Extracted molecule |
total RNA |
| Extraction protocol |
Fresh retinatissues were harvested from 30 day old C57BL/6J mice according to published protocols. 3 retinas were pooled together to form one biological sample. Under a dissecting microscope, spring scissors were used to puncture the eye and remove the cornea, iris, and lens. The remaining eyecup had 4 radial incisions made every 90 degrees, resulting in a flat and open eye cup. The retina was then gently removed using curved tweezers and placed in a 1.5 ml microcentrifuge tube.
10x Genomic 3' mRNA scRNA V2
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
cellranger
Genome_build: GRCm38
Supplementary_files_format_and_content: cellranger matrix output
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| Submission date |
Jul 05, 2020 |
| Last update date |
Sep 29, 2020 |
| Contact name |
Les Kallestad |
| Organization name |
University of California Irvine
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| Department |
Ophthalmology
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| Lab |
Krzysztof Palczewski
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| Street address |
829 Health Sciences Rd
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| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
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| Platform ID |
GPL19057 |
| Series (1) |
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| Relations |
| BioSample |
SAMN15454063 |
| SRA |
SRX8668948 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM4654742_barcodes.tsv.gz |
2.4 Mb |
(ftp)(http) |
TSV |
| GSM4654742_features.tsv.gz |
245.2 Kb |
(ftp)(http) |
TSV |
| GSM4654742_matrix.mtx.gz |
16.9 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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