|
| Status |
Public on Nov 09, 2009 |
| Title |
H3K9me3 PreMegE rep3 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
H3K9me3 miniChIP DNA from PreMegE
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57bl/6
age: 10-12 weeks old
tissue: bone marrow
cell type: Pre-Megakaryocyte/Erythrocyte progenitors (PreMegE)
antibody: H3K9me3
|
| Treatment protocol |
PreMegEs were FACS sorted from the bone marrow of 10-12 week old mice
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The miniChIP method is described in detail in the Supplementary Data file of Weishaupt et al., 2009 (Blood).
|
| Label |
Cy5
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Iceland, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Channel 2 |
| Source name |
Input DNA from PreMegE
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57bl/6
age: 10-12 weeks old
tissue: bone marrow
cell type: Pre-Megakaryocyte/Erythrocyte progenitors (PreMegE)
antibody: none
|
| Treatment protocol |
PreMegEs were FACS sorted from the bone marrow of 10-12 week old mice
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The miniChIP method is described in detail in the Supplementary Data file of Weishaupt et al., 2009 (Blood).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Iceland, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc.,Iceland following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Iceland, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
miniChIP-chip PreMegEs H3K9me3 replicate 3
|
| Data processing |
Arrays were processed using Nimblegen's standard protocol for Nimblescan ChIP data extraction. Arrays were normalized using MA2C and quantile normalization as described in Weishaupt et al., 2009 (Blood).
|
| |
|
| Submission date |
Oct 23, 2009 |
| Last update date |
Jan 03, 2010 |
| Contact name |
Joanne Attema |
| E-mail(s) |
Joanne.Attema@med.lu.se
|
| Organization name |
Lund University
|
| Department |
Immunology
|
| Lab |
BMC D14
|
| Street address |
Tornavagen 10
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL9485 |
| Series (2) |
| GSE18734 |
miniChIP-chip of murine hematopoietic stem cells and progeny with H3K4me3, H3K79me2, H3K9/14ac, H3K27me3, H3K9me3, PolII |
| GSE18737 |
Epigenetic chromatin states uniquely define the developmental plasticity of murine hematopoietic stem cells |
|
