|
| Status |
Public on Sep 08, 2020 |
| Title |
MBAB-Mouse_15_days-CD21-Cd23-CD11b |
| Sample type |
SRA |
| |
|
| Source name |
B cells flow-sorted from the heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
age: 2 weeks old
Sex: pooled male and female
cell type: cardiac B cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Myocardial-associated B cells were isolated by flow sorting using a FACS Aria-II Cell Sorter. Doublets and dead cells were excluded. Neonatal and adult samples were submitted to the same digestion protocol and B cells were sorted on the same day.
10x Genomcis Chromium single cell preparation
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Generation of fastq files via https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest
Alignment to mm10 genome build via https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest
Genome_build: mm10
Supplementary_files_format_and_content: barcodes.tsv, genes.tsv, matrix.mtx
|
| |
|
| Submission date |
Jun 30, 2020 |
| Last update date |
Sep 08, 2020 |
| Contact name |
Luigi Adamo |
| E-mail(s) |
ladamo@wustl.edu
|
| Phone |
314-273-7987
|
| Organization name |
Washington University In St Louis
|
| Department |
Internal Medicine - Cardiovascular Division
|
| Street address |
660 South Euclid Ave
|
| City |
St Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE153568 |
Transcriptional profile of B cells isolated from neonatal and adult hearts |
|
| Relations |
| BioSample |
SAMN15407962 |
| SRA |
SRX8641932 |
