|
| Status |
Public on Aug 29, 2022 |
| Title |
IL-2 KO replicate 3 |
| Sample type |
SRA |
| |
|
| Source name |
Thymic regulatory T cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Thymic regulatory T cells
sorting: CD4+CD8-Thy1.1+GFP+
strain/genotype: Rag2-Gfp Foxp3-Thy1a Kaa Tcrb-transgenic Tcra+/null Il2null B6
age: 3 weeks
|
| Growth protocol |
Sorting
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by Nucleospin RNA XS (Macherey-Nagel)
Libraries for TCR-sequencing (TCR-seq), the cDNA synthesis and library preparation were performed as previously described (Feng et al., 2015), and was adjusted to our different conditions.) the RNA had a high quality (RIN>8.5)
TCR-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
High quality selection with pRESTO-FilterSeq
Indexing and annotation UMI pRESTO-MaskPrimers and PairSeq
Construction concensus pRESTO-BuildConsensus
Aligned F and R pRESTO-AssemblePairs
Aligment to TCR end table export MiXCR-align-assemble-exportClones
Genome_build: mm10
Supplementary_files_format_and_content: Number of UMI and VJ segments, Delimited tab text
|
| |
|
| Submission date |
Jun 29, 2020 |
| Last update date |
Aug 30, 2022 |
| Contact name |
Joost Van Meerwijk |
| E-mail(s) |
Joost.van-Meerwijk@inserm.fr
|
| Phone |
0562748381
|
| Organization name |
INSERM
|
| Department |
CPTP U1043
|
| Lab |
Joost Van Meerwijk Eq1
|
| Street address |
CHU Purpan BP 3028
|
| City |
Toulouse |
| State/province |
HG |
| ZIP/Postal code |
31024 |
| Country |
France |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE153484 |
IL-2 and IL-15 drive intrathymic development of distinct periphery-seeding CD4+Foxp3+ regulatory T lymphocytes |
|
| Relations |
| BioSample |
SAMN15398609 |
| SRA |
SRX8632353 |
