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Sample GSM461176 Query DataSets for GSM461176
Status Public on Oct 09, 2009
Title S2_DRSC_Untreated-1
Sample type SRA
 
Source name S2_DRSC_Untreated
Organism Drosophila melanogaster
Characteristics cell line: S2-DRSC
Treatment protocol Cells were treated with dsRNA to the indicated gene
Growth protocol Schneider's medium + 10% heat-inactivated FCS
Extracted molecule total RNA
Extraction protocol Oligo-dT selected, double stranded cDNA, Single Read Sequencing
TRIzol Dnase Rneasy
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer II
 
Description dsRNA treatment: Untreated
read length: 45
library_source: RNA
library_selection: RT and size selection
Data processing Reads were aligned to the reference genome and a splice junction database to identify uniquely mapping reads and the data converted to GFF files and .bedgraph files which indicate the number of reads that map to each base in the genome.
 
Submission date Oct 09, 2009
Last update date May 15, 2019
Contact name Brenton R Graveley
E-mail(s) graveley@uchc.edu
Phone 860-679-2090
Organization name University of Connecticut Health Center
Department Genetics and Genome Sciences
Street address 400 Farmington Avenue
City Farmington
State/province CT
ZIP/Postal code 06030-3301
Country USA
 
Platform ID GPL9061
Series (1)
GSE18508 modENCODE Drosophila RNA Binding Protein RNAi RNA-Seq Studies
Relations
Reanalyzed by GSM3274546
SRA SRX014458
BioSample SAMN00006272

Supplementary file Size Download File type/resource
GSM461176_Untreated-1_all.bedgraph.gz 57.7 Mb (ftp)(http) BEDGRAPH
GSM461176_Untreated-1_all.gff.gz 374.1 Mb (ftp)(http) GFF
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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