|
| Status |
Public on May 31, 2024 |
| Title |
sample.4_Young ANKO1 |
| Sample type |
SRA |
| |
|
| Source name |
Young ANKO_subcutaneous WAT
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
genotype/variation: ANKO (adipocyte specific Nampt knockout)
age: young (2 month-old)
tissue: Subcutaneous white adipose tissue
|
| Treatment protocol |
Mice were kept at room temperature under a normal regular-chow fed condition.
|
| Growth protocol |
ANKO mice were generated by using adiponectin-Cre transgenic mice and Nampt flox mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Subcutaneous white adipose tissue samples were removed, flash frozen by liquid nitrogen, and RNA was harvested using Qiazol Reagent with Rneasy Mini Kit (Qiagen).
Ribo-Zero Gold kitÂ
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
4.CATAGTCACA-ACGAGGACCA.H7M5YDSXY_CATAGTCACA-ACGAGGACCA_S28_L003
|
| Data processing |
Basecalling with Illumina RTA 1.9
Demultiplexing with custom demultiplexer written in python
Technical replicate fastq files were combined and aligned with STAR 2.0.4b
Gene counts dervied with Subread:featureCount 1.45 and annotated with Ensembl Biomart.
Genome_build: Ensembl GRCm38.76
Supplementary_files_format_and_content: Gene counts in tab-delimitted text MS Excel format
|
| |
|
| Submission date |
Jun 08, 2020 |
| Last update date |
May 31, 2024 |
| Contact name |
Jun Yoshino |
| E-mail(s) |
jyoshino@med.shimane-u.ac.jp
|
| Organization name |
Faculty of Medicine, Shimane University
|
| Street address |
89-1 Enya-cho
|
| City |
Izumo |
| State/province |
Shimane |
| ZIP/Postal code |
693-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE152067 |
Adipocyte-specific inactivation of NAMPT, a key NAD+ biosynthetic enzyme, causes a metabolically-unhealthy lean phenotype in female mice during aging |
|
| Relations |
| BioSample |
SAMN15182107 |
| SRA |
SRX8504762 |
