|
| Status |
Public on Dec 05, 2015 |
| Title |
JSC1_mir221_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
JSC1_mir221
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: JSC1
hairpin: hsa-miR-221
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol purification of total RNA
|
| Label |
Cy5
|
| Label protocol |
Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
|
| |
|
| Channel 2 |
| Source name |
Stratagene reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample: reference RNA
hairpin: none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol purification of total RNA
|
| Label |
Cy3
|
| Label protocol |
Standard Agilent 4x44k protocol (amplification and labelling of 500 ng of total RNA)
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed for 17 hrs at 65°C
|
| Scan protocol |
Arrays were scanned on an Agilent DNA microarray scanner. Images were analyzed and data were extracted using the standard procedures of Agilent Feature Extraction software (v9.5.3.1).
|
| Description |
Sample used in miR221 experiment
|
| Data processing |
Scale normalisation across arrays (R/BioConductor), with samples 1-6 and 7-20 being normalised separately.
|
| |
|
| Submission date |
Oct 06, 2009 |
| Last update date |
Dec 05, 2015 |
| Contact name |
Dan Frampton |
| Organization name |
UCL
|
| Department |
Infection
|
| Lab |
Kellam
|
| Street address |
Gower Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE18438 |
Over-expression of hsa-miR-221 in JSC1 cells and identification of miRNA targets expressed in a panel of B-cell lines |
| GSE18439 |
Transcription repression of miR-221 leads to p27 expression in PEL |
|
