|
| Status |
Public on Jan 01, 2010 |
| Title |
Pancreas of PND3 Glis3 mutant mice Vs. WT |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Glis3 mutant pancreas, PND3 mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: post natal day (PND) 3
tissue: pancreas
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA easy mini kit with on-column DNAseI treatment
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit protocol. Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Channel 2 |
| Source name |
WT Pancreas, PND3 mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: post natal day (PND) 3
tissue: pancreas
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA easy mini kit with on-column DNAseI treatment
|
| Label |
Cy5
|
| Label protocol |
Total RNA was amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit protocol. Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| |
| Hybridization protocol |
For each two color comparison, 750ng of each Cy3 and Cy5 labeled cRNAs were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven using the Agilent 60-mer ol
|
| Scan protocol |
Slides were washed as indicated in the Agilent 60-mer oligo microarray processing protocol and then scanned with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software, using defaults for all parameters.
|
| Description |
Sample contains RNA from 2 different Glis3 mutant mice
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v8.1), using defaults for all parameters and loaded into the Rosetta Resolver® system (v6.0) (Rosetta Biosoftware, Kirkland, WA).
|
| |
|
| Submission date |
Sep 18, 2009 |
| Last update date |
Sep 22, 2009 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE18172 |
T.F. Glis3: a novel critical player in the regulation of pancreatic beta-cell development and insulin gene expression |
|
