Cultured in media containing sodium chloride at 8.6 grams/liter.
Growth protocol
Z. mobilis ZM4 was obtained from the American Type Culture Collection (ATCC31821) and cultured in RM medium at 30°C.
Extracted molecule
total RNA
Extraction protocol
RNA was isolated from samples which were harvested by centrifugation. The TRIzol reagent (Invitrogen, Carlsbad, CA) was used to extract total cellular RNA.
Label
Cy3
Label protocol
Nimblegen internal protocols were followed
Hybridization protocol
Nimblegen internal protocols were followed
Scan protocol
Nimblegen internal protocols were followed
Description
2166502
Data processing
Data was normalized using the Loess normalization analytical process in the JMP Genomics 4.0 Package from SAS Institute
