|
| Status |
Public on May 31, 2020 |
| Title |
BC5 |
| Sample type |
RNA |
| |
|
| Source name |
BC5
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Paired primary breast cancer
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from FFPE sections using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) as per Agilent one-color (Agilent) labeling kit.
|
| |
|
| Hybridization protocol |
Labeled samples were hybridized to the Agilent 8 × 60k v16 miRNA microarray chip
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting
|
| Description |
microRNA expression of primary breast cancer.
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 11.0 (Agilent) using default parameters. Data were analyzed using Agilent GeneSpring X software
|
| |
|
| Submission date |
Apr 17, 2020 |
| Last update date |
Jun 01, 2020 |
| Contact name |
Nehad M Alajez |
| E-mail(s) |
nalajez@gmail.com
|
| Organization name |
King Saud University College of Medicine
|
| Department |
Anatomy
|
| Lab |
Stem Cell Unit
|
| Street address |
1st floor College of Medicne
|
| City |
Riyadh |
| ZIP/Postal code |
11461 |
| Country |
Saudi Arabia |
| |
|
| Platform ID |
GPL21576 |
| Series (2) |
| GSE148846 |
microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients |
| GSE148848 |
microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors |
|
