|
| Status |
Public on Sep 26, 2022 |
| Title |
HC28 Bcell |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Healthy control
cell type: Bcell
date measured: 14.01.22
age: 37
Sex: F
sex (1 vs 2): 2
|
| Treatment protocol |
Fresh peripheral blood from SLE patients and healthy volunteers was collected and T, B, monocyte, mDC, pDC, naiveB, memoryB and PBMC were isolated at spontaneous state.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNAqueous Micro Kit (Thermo Fisher Scientific/Ambion, USA) with RNase-Free DNase Set (Qiagen). RNA Integrity Number (RIN) value was measured using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). RNA samples with RIN ≥7.0 were used.
|
| Label |
Cy3
|
| Label protocol |
Total RNA from a human sample were amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit and labeled with Cy3 respectively according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Labeled samples were hybridized to microarray G4110B Human 1A Microarray (V2) for 17hr, 65 deg C.
|
| Scan protocol |
Gene expression pixel intensities were extracted by scanning the microarrays with an Agilent Scanner.
|
| Data processing |
Data processing and trimming were performed with GeneSpring ver. 12.6.1.
|
| |
|
| Submission date |
Apr 13, 2020 |
| Last update date |
Sep 26, 2022 |
| Contact name |
Kenji Okuma |
| E-mail(s) |
kenji.okuma@astellas.com
|
| Phone |
+81-75-366-7462
|
| Organization name |
Astellas Pharma Inc.
|
| Street address |
53, Shogoin Kawara-cho, Sakyo-ku, Medical Innovation Center
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE148601 |
mRNA expression analysis of several cell subset from peripheral blood of SLE patient and Healthy Control |
|
