|
| Status |
Public on Sep 20, 2020 |
| Title |
patient rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Monocytes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PBMC
disease state: Vogt-Koyanagi-Harada
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Fresh heparinized venous blood samples from healthy donors and VKH patients were processed within 2 hours of collection. PBMCs were isolated from fresh blood using Ficoll density gradient centrifugation at 500g for 30min at room temperature (25oC). Single-cell suspensions were processed by pan monocyte isolation kit according to the manufacturers’ instructions (Thermo Fisher Scientific, catalog no. 8804-6837-74) in order to enrich monocytes as many as possible for single-cell RNA sequencing.
Chromiun Single Cell 3' Reagent v2 or v3
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Cellranger v3.1 was used for demultiplexing, aligment and quantification.
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited text files include feature name or barcode of each sample, and mtx files containing expression value of each gene in each cell of each sample.
|
| |
|
| Submission date |
Apr 02, 2020 |
| Last update date |
Sep 20, 2020 |
| Contact name |
yuhua xiao |
| E-mail(s) |
xiaoyh26@mail2.sysu.edu.cn
|
| Phone |
18728192414
|
| Organization name |
Sun Yat-sen University
|
| Department |
zhongshan Ophthalmic center
|
| Street address |
jinsui load
|
| City |
guangzhou |
| State/province |
guangdong province |
| ZIP/Postal code |
510627 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE148020 |
Genetic landscape and autoimmunity of monocytes in developing Vogt-Koyanagi-Harada disease |
|
| Relations |
| BioSample |
SAMN14530192 |
| SRA |
SRX8049104 |
