|
| Status |
Public on Dec 01, 2009 |
| Title |
Shoot_+Pi_sRNASeq |
| Sample type |
SRA |
| |
|
| Source name |
shoot
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: Col0
|
| Treatment protocol |
Seventeen-day-old seedlings were subjected for phosphate starvation treatment by transferring them from 250 μM KH2PO4 (+Pi) into 0 μM KH2PO4 (-Pi) nutrient solution. Control samples were maintained under 250 μM KH2PO4 (+Pi) nutrient solution. Roots and shoots were collected separately after 5-day treatment.
|
| Growth protocol |
Nine-day-old Arabidopsis seedlings grown on half-strength modified Hoagland nutrient solution containing 250 μM KH2PO4 (+Pi) and 1% sucrose were transferred to a hydroponic culture system with half-strength modified Hoagland nutrient solution containing 250 μM KH2PO4 for additional growth of 8 days. All plants were grown at 22oC under a 16-hr photoperiod with cool fluorescent light at 100 to 150 μE.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the use of TRIzol reagent (Invitrogen, Carlsbad, CA) following the manufacturer's instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Treatment: 250uM KH2PO4 for 5 days
|
| Data processing |
Processed data files contain the perfectly mapped reads with 5' and 3' adapters removed (reads were mapped on the TAIR8 genome). Same reads are sorted together and the numbers of repetitivity are indicated
|
| |
|
| Submission date |
Aug 20, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Li-Ching Hsieh |
| Organization name |
National Chung Hsing University
|
| Department |
Graduate Institute of Genomics and Bioinformatics
|
| Lab |
Hsieh Lab
|
| Street address |
145 Xingda Rd.
|
| City |
Taichung |
| ZIP/Postal code |
402 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL9062 |
| Series (1) |
| GSE17741 |
Uncovering small RNA-mediated responses to phosphate-deficiency in Arabidopsis by deep sequencing |
|
| Relations |
| SRA |
SRX014817 |
| BioSample |
SAMN00007003 |
