|
| Status |
Public on Dec 21, 2022 |
| Title |
shREN_1bulkRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
pre B cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: pre B cells
genotype: shREN
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PreB cells from WT mice were harvested and transduced with shRNAs targeting either shAlox15b or shAlox12b, fluorescence activated cell sorting of transduced cells were conducted 48h post-infection and RNA was harvested using Trizol reagent.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
shREN_1.fq.gz
|
| Data processing |
Illumina sequencing workflow
Sequenced reads were mapped to GRCm38 whole genome using STAR v2.6.0 .
Transcripts Per Kilobase Million of library size (TPM) were calculated .
Genome_build: GRCm38
Supplementary_files_format_and_content: xlsx file includes TPM values for each Sample
|
| |
|
| Submission date |
Mar 20, 2020 |
| Last update date |
Dec 21, 2022 |
| Contact name |
Xaingyu Pan |
| E-mail(s) |
pangxueyu233@outlook.com
|
| Organization name |
Sichuan University
|
| Department |
State Key Laboratory of Biotherapy, West China Hospital, Sihcuan University.
|
| Lab |
Chen & Liu Lab
|
| Street address |
No.17 Renming road section3
|
| City |
Chengdu, Cihna |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE147264 |
COX-2/PGE2 upregulation contributes to the chromosome 17p-deleted lymphoma |
|
| Relations |
| BioSample |
SAMN14411466 |
| SRA |
SRX7958938 |
