|
| Status |
Public on Sep 21, 2011 |
| Title |
Σ1278b flo8 deleted, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Yeast carrying a deletion in flo8 transformed with empty vector
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: Σ1278b, H-U Moesch
genotype: MATα ura3-52 trp1Δ::hisG leu2Δ::hisG his3Δ::hisG
plasmid: YEpLac195-FLO8
|
| Treatment protocol |
Yeast cultures grown to early stationary phase, cells were seperated from growth medium, washed and mixed with extraction buffer and phenol/chloroform before being frozen in liquid nitrogen
|
| Growth protocol |
Yeast grown in 5ml (test tubes on rotary wheel) synthetic dropout media (YNB) with 2% glucose at 30 degrees Celsius. Total RNA harvested at entry into stationary phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Phenol-Chloroform exctraction with heat treatment (65 degrees Celsius 5 min.). Schmitt et al., 1990, A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res 18: 3091-3092.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Performed according to standard Affymetrix protocol
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip® scanner 3000and the Affymetrix GeneChip® Operating Software (GCOS) version 1.4.
|
| Description |
Gene expression data of yeast grown till early stationary phase in synthetic dropout media
|
| Data processing |
Data normalisation performed with GC-RMA
|
| |
|
| Submission date |
Aug 19, 2009 |
| Last update date |
Sep 23, 2011 |
| Contact name |
Michael Christiaan Bester |
| E-mail(s) |
bester@sun.ac.za
|
| Phone |
+27218084867
|
| Organization name |
Institute for Wine Biotechnology
|
| Lab |
Yeast Lab
|
| Street address |
Victoria street
|
| City |
Stellenbosch |
| ZIP/Postal code |
7600 |
| Country |
South Africa |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE17716 |
Transcription data from Saccharomyces cerevisiae yeast |
|
