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Sample GSM434758 Query DataSets for GSM434758
Status Public on Jan 01, 2010
Title MouseRetina-3mons-2
Sample type RNA
 
Source name Mouse retina, 3months old, pooled sample 2
Organism Mus musculus
Characteristics tissue: Retina
gender: male
age: 3 Months
Extracted molecule total RNA
Extraction protocol Mouse retina was dissected immediately after mouse was sacrified. RNA was isloated using standard RNA extraction protocols (Trizol). RNA's was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 1μg RNA using the One-Color Low RNA Input Linear Amplification kit (Agilent) according to the manufacturer's instructions.Yields of cRNA and the dye-incorporation rate were checked with the NanoDrop ND-1000 Spectrophotometer(NanoDrop Technologies).
 
Hybridization protocol 1.65 μg of Cy3-labelled fragmented cRNA in hybridization buffer was hybridized to Agilent whole Mouse Genome Oligo Microarrays 4X44K for 17 hours at 65°C. After hybridization, microarrays were washed once at room temperature with 6x SSPE Buffer for 1 minute followed by a second wash with preheated 0.06x SSPE Buffer (37°C) for 1 minute. The last washing step was performed with acetonitrile for 30 second.
Scan protocol Slides were scanned on the Agilent's Microarry Scanner system (Agilent Technologies).
Description MouseRetina-3mons-2
Data processing The Agilent Feature Extraction Software (FES) was used to read out and process the microarray image files. The raw data were normalized by dividing the intensity values by their median.
 
Submission date Jul 30, 2009
Last update date Jul 31, 2009
Contact name Heping Xu
E-mail(s) opt090@abdn.ac.uk
Organization name Univeristy of Aberdeen
Department Ophthalmology
Street address Forresterhill
City Aberdeen
ZIP/Postal code AB25 2ZD
Country United Kingdom
 
Platform ID GPL4134
Series (1)
GSE17423 Age-related para-inflammation in the neuroretina of the mouse

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
12 0.013136
13 1.375515
14 0.039741
15 7.691991
16 0.636508
18 0.013382
19 0.398168
20 0.054195
21 0.536972
22 4.967107
23 0.013414
24 20.135195
25 12.929463
26 21.125887
27 0.784221
28 6.741549
29 0.757481
30 0.10798
31 0.356027
32 0.188827

Total number of rows: 43379

Table truncated, full table size 631 Kbytes.




Supplementary file Size Download File type/resource
GSM434758.txt.gz 8.0 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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