|
| Status |
Public on Sep 23, 2020 |
| Title |
SMG-WT |
| Sample type |
SRA |
| |
|
| Source name |
Primary Mouse Epithelial Progenitor Cells
|
| Organism |
Mus musculus |
| Characteristics |
age: Adult (12wk)
tissue: Salivary Gland
genotype/variation: p63 WildType mouse
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole mouse Salivary gland was dissociated into single cells and sujected to single-cell sequencing
Chromimum Single Cell 3' v2 (10x Genomics)
TruSeq Stranded RNA (illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
scRNA-Sequencing
|
| Data processing |
Illumina RTA 2.2.68 Basecaller
Cellranger 2.1.0 mkoutput with Bcl2Fastq 2.19.1
Cellranger 2.1.0 Count against mm10 genome to produce count matrix
Seurat 3.1.2 used for marker gene analysis and visualization
Genome_build: mm10
Supplementary_files_format_and_content: CSV File containing UMI corrected read counts
|
| |
|
| Submission date |
Feb 13, 2020 |
| Last update date |
Sep 23, 2020 |
| Contact name |
Jonathan E Bard |
| E-mail(s) |
jbard@buffalo.edu
|
| Organization name |
University at Buffalo
|
| Department |
Genomics and Bioinformatics Core
|
| Street address |
701 Ellicott Street
|
| City |
Buffalo |
| State/province |
New York |
| ZIP/Postal code |
14203 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE145267 |
p63 Maintains the Proliferative Potential of Salivary Gland Stem/Progenitor Cells by Regulating Follistatin, a Key Mediator of TGF-b/Activin Signaling [scRNA-seq] |
| GSE145268 |
p63 Maintains the Proliferative Potential of Salivary Gland Stem/Progenitor Cells by Regulating Follistatin, a Key Mediator of TGF-b/Activin Signaling |
|
| Relations |
| BioSample |
SAMN14096857 |
| SRA |
SRX7719803 |
