|
Status |
Public on Jan 27, 2021 |
Title |
Meninges, Young |
Sample type |
SRA |
|
|
Source name |
Dural meninges
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J tissue: Dural meninges cell type: Endothelial and mural age: 2 months Sex: Male
|
Treatment protocol |
not applicable
|
Growth protocol |
not applicable
|
Extracted molecule |
total RNA |
Extraction protocol |
10x Genomics Chromium single cell preparation NGS Illumina Next Seq
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
Dural endothelial (CD45-CD31+CD13-) and mural cells (CD34-CD13+CD31-) were sorted from 5 mice
|
Data processing |
Illumina bcl2fastq software used for basecalling UMI count matrices generated using the Cellranger count pipeline. Fastq files from each run were specified using the --fastqs flag The cellranger count pipeline, resulted in one count matrix for each of our sample conditions (brain old, brain young, meninges old, meninges young) Genome_build: mm10 Supplementary_files_format_and_content: tar archive containing the raw UMI count matrices for each sample as well as matrices with cell barcodes and gene symbols.
|
|
|
Submission date |
Jan 23, 2020 |
Last update date |
Jan 27, 2021 |
Contact name |
Jonathan Kipnis |
Organization name |
Washington University in Saint Louis
|
Department |
Pathology and Immunology
|
Lab |
Kipnis Lab
|
Street address |
4515 McKinley Ave
|
City |
Saint Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE144174 |
Cellular and molecular characterization of the dural sinuses as a neuroimmune interface [vasculature] |
GSE144175 |
Cellular and molecular characterization of the dural sinuses as a neuroimmune interface |
|
Relations |
BioSample |
SAMN13910675 |
SRA |
SRX7625311 |