|
| Status |
Public on Jan 27, 2021 |
| Title |
Meninges, Young |
| Sample type |
SRA |
| |
|
| Source name |
Dural meninges
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: Dural meninges
cell type: Endothelial and mural
age: 2 months
Sex: Male
|
| Treatment protocol |
not applicable
|
| Growth protocol |
not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10x Genomics Chromium single cell preparation
NGS Illumina Next Seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Dural endothelial (CD45-CD31+CD13-) and mural cells (CD34-CD13+CD31-) were sorted from 5 mice
|
| Data processing |
Illumina bcl2fastq software used for basecalling
UMI count matrices generated using the Cellranger count pipeline. Fastq files from each run were specified using the --fastqs flag The cellranger count pipeline, resulted in one count matrix for each of our sample conditions (brain old, brain young, meninges old, meninges young)
Genome_build: mm10
Supplementary_files_format_and_content: tar archive containing the raw UMI count matrices for each sample as well as matrices with cell barcodes and gene symbols.
|
| |
|
| Submission date |
Jan 23, 2020 |
| Last update date |
Jan 27, 2021 |
| Contact name |
Jonathan Kipnis |
| Organization name |
Washington University in Saint Louis
|
| Department |
Pathology and Immunology
|
| Lab |
Kipnis Lab
|
| Street address |
4515 McKinley Ave
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE144174 |
Cellular and molecular characterization of the dural sinuses as a neuroimmune interface [vasculature] |
| GSE144175 |
Cellular and molecular characterization of the dural sinuses as a neuroimmune interface |
|
| Relations |
| BioSample |
SAMN13910675 |
| SRA |
SRX7625311 |
