|
| Status |
Public on Aug 21, 2009 |
| Title |
AG09699 |
| Sample type |
RNA |
| |
|
| Source name |
AG09699
|
| Organism |
Homo sapiens |
| Characteristics |
age: 46
sex: M
race: black
tissue.origin: Skin
|
| Biomaterial provider |
coriell
|
| Growth protocol |
fibroblasts were cultured in DMEM supplemented with 10% fetal calf serum, L-glutamine, penicillin, and streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy protocol and reagents for extraction of total RNA
|
| Label |
biotin
|
| Label protocol |
Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
|
| |
|
| Hybridization protocol |
Microarray = Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
|
| Scan protocol |
Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
|
| Description |
AG09699 fibroblast cell line
|
| Data processing |
MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
|
| |
|
| Submission date |
Jul 09, 2009 |
| Last update date |
Aug 18, 2009 |
| Contact name |
Ben S. Wittner |
| E-mail(s) |
wittner.ben@mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Cancer Research
|
| Lab |
Lawrence
|
| Street address |
149 13th Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE17032 |
Expression data from human fibroblasts |
|
