|
| Status |
Public on Feb 24, 2020 |
| Title |
7-LT-localized tumor |
| Sample type |
RNA |
| |
|
| Source name |
Solid pseudopapillary neoplasm (SPN) of pancreas
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: LT-localized tumor
sample type: Formalin-fixed paraffin-embedded blocks (FFPE Blocks)
|
| Growth protocol |
tissue protocol: Formalin-fixed paraffin-embedded blocks of SPN specimens were obtained from the archives of the Institute of Pathology. Samples were reviewed, and ten micrometer thick sections were cut from the tumor block, and transferred on to glass slides. A single glass slide containing a block sample was stained with hematoxylin and eosin, and tumor margins were marked by the pathologist (EB). Microdissection was performed manually to extract tumoral tissue using a sharp scalpel.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using miRNeasy Formalin-fixed paraffin-embedded kit (Qiagen, Hilden, Germany), according to manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Genisphere FlashTag Biotin Labeling Assay according to the manufacturer's protocol
|
| |
|
| Hybridization protocol |
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
|
| Scan protocol |
GenePix 4000B array scanner, according to the manufacturer's protocol
|
| Data processing |
Gene-level analyses was performed. RMA normalization, summarization by mean, all were done by Partek Genomics suite (version 6.5) Copyright ©
|
| |
|
| Submission date |
Nov 20, 2019 |
| Last update date |
Feb 25, 2020 |
| Contact name |
Metsada Pasmanik-Chor |
| E-mail(s) |
metsada@tauex.tau.ac.il
|
| Organization name |
Tel Aviv University
|
| Department |
Biology
|
| Lab |
Bioinformatics Unit
|
| Street address |
Ramat Aviv
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE140719 |
Micro-RNA Expression Patterns Predict Metastatic Spread in Solid Pseudopapillary Neoplasms of the Pancreas |
|
