|
| Status |
Public on Jun 10, 2010 |
| Title |
E2F2-/- E17.5_15 |
| Sample type |
RNA |
| |
|
| Source name |
Lens control sample at E17.5
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lens
cell type: epithelial cells
age: E17.5
developmental stage: embryo
genotype/variation: control
|
| Treatment protocol |
Both lenses from the mouse were dissected with 18-gauge needles under a microscope and immediately transferred to TRIzol. Lenses were then homogenized and processed for RNA extraction.
|
| Growth protocol |
Embryos or pups were collected from timed pregnancies in which parental contribution would result in offspring with the E2F2-/- genotype or multiple combinations of wild type or null alleles of E2F1, E2F2, and E2F3 in the lens of the eye.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
All steps of GeneChip hybridization were performed by the MicroArray Shared Resource (MASR) for the OSU Comprehensive Cancer Center (OSUCCC) according to standard operating procedures (SOP) following the recommendations provided by Affymetrix.
|
| Scan protocol |
Scanning was performed by the MicroArray Shared Resource (MASR) for the OSU Comprehensive Cancer Center (OSUCCC) according to standard operating procedures (SOP) following the recommendations provided by Affymetrix.
|
| Description |
Gene expression data from lenses microdissected from E2F2-/- E17.5 murine embryos
|
| Data processing |
The data were quantile normalized by RMAExpress using background adjustment and median polish summarization. Signal intensities provided in the matrix table were exported in log scale. Data were subsequently imported into the BRB Array Tools plugin for Microsoft Excel for analysis.
|
| |
|
| Submission date |
Jun 10, 2009 |
| Last update date |
Jun 10, 2009 |
| Contact name |
Pamela L. Wenzel |
| E-mail(s) |
pamela.wenzel@childrens.harvard.edu
|
| Phone |
617-919-2016
|
| Fax |
617-730-0222
|
| Organization name |
Children's Hospital Boston
|
| Department |
Hematology/Oncology
|
| Lab |
George Daley
|
| Street address |
300 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE16533 |
Expression data from lenses triply deficient for E2F1, E2F2, and E2F3 transcription factors |
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