|
| Status |
Public on Oct 21, 2020 |
| Title |
AML cells, Trib1 hi 4 |
| Sample type |
RNA |
| |
|
| Source name |
AML cell line expressing Hoxa9 and Trib1
|
| Organism |
Mus musculus |
| Characteristics |
cell line: AML cell line
genotype: Hoxa9 and Trib1
|
| Treatment protocol |
No treatment was provided.
|
| Growth protocol |
Trib1 hi and null cells were grown in IMDM supplemented with 10% fetal bovine serum, and 5 ng/ml IL-3. Mouse AML cells developed in the Trib1 homozygous knock-out mouse.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
|
| Description |
Gene expression data from mouse AML
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 31, 2019 |
| Last update date |
Oct 21, 2020 |
| Contact name |
Takuro Nakamura |
| E-mail(s) |
takuro-ind@umin.net
|
| Phone |
81-3-3570-0462
|
| Organization name |
Japanese Foundation for Cancer Research
|
| Department |
The Cancer Institute
|
| Lab |
Carcinogenesis
|
| Street address |
3-8-31 Ariake, Koto-ku
|
| City |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE139641 |
Trib1 promotes acute myeloid leukemia progression by modulating transcriptional programs of Hoxa9 [array] |
| GSE140315 |
Trib1 promotes acute myeloid leukemia progression by modulating transcriptional programs of Hoxa9 |
|
