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Sample GSM4145062 Query DataSets for GSM4145062
Status Public on Oct 21, 2020
Title AML cells, Trib1 hi 4
Sample type RNA
 
Source name AML cell line expressing Hoxa9 and Trib1
Organism Mus musculus
Characteristics cell line: AML cell line
genotype: Hoxa9 and Trib1
Treatment protocol No treatment was provided.
Growth protocol Trib1 hi and null cells were grown in IMDM supplemented with 10% fetal bovine serum, and 5 ng/ml IL-3. Mouse AML cells developed in the Trib1 homozygous knock-out mouse.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
Description Gene expression data from mouse AML
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Oct 31, 2019
Last update date Oct 21, 2020
Contact name Takuro Nakamura
E-mail(s) takuro-ind@umin.net
Phone 81-3-3570-0462
Organization name Japanese Foundation for Cancer Research
Department The Cancer Institute
Lab Carcinogenesis
Street address 3-8-31 Ariake, Koto-ku
City Tokyo
ZIP/Postal code 135-8550
Country Japan
 
Platform ID GPL11180
Series (2)
GSE139641 Trib1 promotes acute myeloid leukemia progression by modulating transcriptional programs of Hoxa9 [array]
GSE140315 Trib1 promotes acute myeloid leukemia progression by modulating transcriptional programs of Hoxa9

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 318.716
AFFX-BioB-M_at 358.68027
AFFX-BioB-3_at 323.62378
AFFX-BioC-5_at 848.1132
AFFX-BioC-3_at 1085.846
AFFX-BioDn-5_at 2251.1707
AFFX-BioDn-3_at 4200.1235
AFFX-CreX-5_at 10638.955
AFFX-CreX-3_at 11825.814
AFFX-DapX-5_at 289.7808
AFFX-DapX-M_at 1143.7158
AFFX-DapX-3_at 1454.3003
AFFX-LysX-5_at 3.5334673
AFFX-LysX-M_at 3.9169896
AFFX-LysX-3_at 6.147279
AFFX-PheX-5_at 6.4106913
AFFX-PheX-M_at 6.388445
AFFX-PheX-3_at 8.047354
AFFX-ThrX-5_at 5.14008
AFFX-ThrX-M_at 5.575162

Total number of rows: 45141

Table truncated, full table size 1056 Kbytes.




Supplementary file Size Download File type/resource
GSM4145062_hi1-TKO-H+T_4.ga.cel.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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