|
| Status |
Public on Aug 24, 2021 |
| Title |
Murine epididymal adipose tissue from (rAAV-Lmo3) transduced mice (pool of n=2 mice) after 12 weeks of HFD, 3 |
| Sample type |
RNA |
| |
|
| Source name |
Murine epididymal adipose tissue from (rAAV-Lmo3) transduced mice (pool of n=2 mice) after 12 weeks of HFD, replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
group: (rAAV-Lmo3) transduced
Sex: male
strain: C57BL6/J
tissue: Murine epididymal adipose tissue
|
| Treatment protocol |
Visceral (epididymal) adipose tissue from obese rAAV-LacZ (control) or rAAV-Lmo3 (experimental) transduced C57BL6/J mice was collected after 12 weeks of high fat diet (HFD).
|
| Growth protocol |
Animals were exposed to a High Fat Diet (Reseach Diets cat.No. D12492) at an age of 6 weeks for a total of 12 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol method.
|
| Label |
Biotin
|
| Label protocol |
100ng of total RNA were amplified and labeled using the GeneChip® WT PLUS Reagent Kit (Catalog Number 902281, Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® Mouse Gene 1.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual (Fluidics Protocol FS450_0007)
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
|
| Data processing |
RMA (Robust Multiarray average) Signal extraction using Affymetrix Expression Console Version 1.1
|
| |
|
| Submission date |
Oct 21, 2019 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Markus Jeitler |
| E-mail(s) |
markus.jeitler@meduniwien.ac.at
|
| Organization name |
Medical University Vienna
|
| Street address |
Lazarettgasse 14
|
| City |
Vienna |
| ZIP/Postal code |
A-1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE139162 |
LMO3 reprogramms viseral adipocyte metabolism during obesity [Mouse] |
| GSE139163 |
LMO3 reprogramms viseral adipocyte metabolism during obesity |
|
