|
| Status |
Public on Oct 17, 2020 |
| Title |
EZH1-0hour-R1 |
| Sample type |
SRA |
| |
|
| Source name |
HUVEC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HUVEC
antibody: EZH1
time point: 0hour
|
| Treatment protocol |
50ng/ml VEGFa was used to treat serum-starved HUVEC cells. For modified RNA experiments, 0.5ug/ml modified RNA expressing GFP and ETS1 was used. For siRNA experiment, 10ng/ml siRNA was used.
|
| Growth protocol |
Primary HUVEC cells purchased from Lonza within 3-6 passages were grown in EBM2+1% FBS or EGM2 medium (Lonza)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP-seq: Chromatin DNA was extracted from sonicated nuclei and further selectively accumulated by ChiP-specific antibody. RNA-seq: total RNA were purified by Qiagen RNeasy column.
Novaseq
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
ChIP-seq reads were aligned to the hg19 human genome assembly using bowtie2 with the following configurations: -p 1
Mapped reads were selected for further analysis using SAMTools.
ngsplot was used for visualization, IGVTools was used to make TDF files.
Genome_build: hg19
Supplementary_files_format_and_content: TDF files.
|
| |
|
| Submission date |
Oct 18, 2019 |
| Last update date |
Oct 21, 2019 |
| Contact name |
bing zhang |
| E-mail(s) |
bingzhang@sjtu.edu.cn
|
| Organization name |
Shanghai Jiao Tong University
|
| Department |
Shanghai Center for Systems Biomedicine
|
| Lab |
Bing Zhang Lab
|
| Street address |
800 Dong Chuan Road
|
| City |
Minhang |
| State/province |
Shanghai |
| ZIP/Postal code |
200240 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE139077 |
Two faces of bivalent domain regulate VEGFA responsiveness and angiogenesis |
|
| Relations |
| BioSample |
SAMN13054597 |
| SRA |
SRX7018711 |
