|
| Status |
Public on Jun 01, 2010 |
| Title |
Nipponbare_shoot 3-4weeks, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
shoot 3-4weeks
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
tissue: shoot
cultivar: Nipponbare
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated and purified by RNeasy Plant Mini Kit (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
According to Affymetrix standard procedure, 10 micrograms of fragmented cRNA were hybridized to an array for 16-18 hours at 45°C with constant rotation at 60 rpm. After hybridization, the array was washed and stained using an Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneArray 3000 scanner.
|
| Description |
Gene expression data from rice shoot
|
| Data processing |
The data were analyzed with GCOS 1.3 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 29, 2009 |
| Last update date |
Jun 01, 2010 |
| Contact name |
Nori Kurata |
| E-mail(s) |
nkurata@lab.nig.ac.jp
|
| Phone |
+81-55-981-6808
|
| Fax |
+81-55-981-6879
|
| Organization name |
National Institute of Genetics
|
| Lab |
Plant Genetics Lab.
|
| Street address |
1111 Yata
|
| City |
Mishima |
| ZIP/Postal code |
411-8540 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE16341 |
A simple optimization can improve the performance of single feature polymorphism detection by Affymetrix expression array: Transcript and Genome Hybridizations |
|
