total RNA was isolated and purified by RNeasy Plant Mini Kit (QIAGEN)
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol
According to Affymetrix standard procedure, 10 micrograms of fragmented cRNA were hybridized to an array for 16-18 hours at 45°C with constant rotation at 60 rpm. After hybridization, the array was washed and stained using an Affymetrix Fluidics Station.
Scan protocol
GeneChips were scanned using Affymetrix GeneArray 3000 scanner.
Description
Gene expression data from rice young panicle
Data processing
The data were analyzed with GCOS 1.3 using Affymetrix default analysis settings and global scaling as normalization method.
