|
| Status |
Public on Sep 10, 2019 |
| Title |
WT Treg replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Foxp3+ YFP-sorted regulatory T cell
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wild type
cell type: Foxp3+ YFP-sorted regulatory T cell
|
| Treatment protocol |
Foxp3+ regulatory T cells were isolated using FACS sorting via YFP (after CD4+ column enrichment).
|
| Growth protocol |
Floxed Rcor1 mice (Jackson Laboratory #025877) were mated with Foxp3YFPcre mice (Rubtsov et al. Immunity 2008) to generate conditional Rcor1-knockout mice lacking Rcor1 in Foxp3+ Treg. Foxp3YFPcre mice were used as control. We used male mice 6-8 weeks of age in this study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNeasy kits (QIAGEN), and RNA integrity and quantity were analyzed by NanoDrop ND-1000 and Nanochip 2100 Bioanalyzer (Agilent Technologies)
NEBNext® Ultra™ II RNA Library Prep was used according to the manufacturers instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Cre_2
|
| Data processing |
Base calling, aligment, filtering, peak calling, normalized abundance measurements, by Novogene company.
remove adapter, low quality, N reads
Supplementary_files_format_and_content: fq; BAM (mapping)STAR software to alignment for RNA-seq sequencing data analysis, using the MM10 genome
Genome_build: MM10
Supplementary_files_format_and_content: Rcor1_Hancock_normalized_data.xls reports normalized abundance measurements.
|
| |
|
| Submission date |
Sep 09, 2019 |
| Last update date |
Sep 10, 2019 |
| Contact name |
Wayne W Hancock |
| Organization name |
CHOP/UPenn
|
| Street address |
916B ARC, 3615 Civic Ctr Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104-4318 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE137137 |
Whole transcriptome sequencing of Treg with and without Rcor1 deletion |
|
| Relations |
| BioSample |
SAMN12717927 |
| SRA |
SRX6821376 |
