|
| Status |
Public on Jun 14, 2009 |
| Title |
T244 Co 24h rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mesenchymal stem cell, inactivated LRP5, control medium, 24 hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: T244
cell type: mesenchymal stem cell
form of overexpressed lrp5: inactivated
treatment: control conditioned medium
treatment time: 24 hr
|
| Treatment protocol |
Cells were treated with 50% control condition medium or Wnt3a condition medium for 1 hour or 24 hours.
|
| Growth protocol |
Cells were cultured in minimum essential medium (MEM, Invitrogen) supplemented with 10% fetal bovine serum (FBS, PAA laboratories), and 1% penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by the RNeasy mini kit (Qiagen), followed by DNase I treatment with the same kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays. Quality control was performed with the Agilent Bioanalyzer.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
hMSC immortalized by human telomerase.
replicate 3 of 3.
|
| Data processing |
The data were normalised using cubic spline normalisation with Illumina Beadstudio software.
Non-normalized data are available in the 'GSE16186_non-normalized_data.txt' file, which is linked to the GSE16186 record as a supplementary file.
|
| |
|
| Submission date |
May 20, 2009 |
| Last update date |
May 20, 2009 |
| Contact name |
na li |
| E-mail(s) |
na.li@mdc-berlin.de
|
| Phone |
0049-30-94063043
|
| Organization name |
MAX-DELBRÜCK-CENTRUM FÜR MOLEKULARE MEDIZIN (MDC)
|
| Lab |
AG chen
|
| Street address |
Robert-Rössle-Straße 10
|
| City |
berlin |
| ZIP/Postal code |
13125 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5104 |
| Series (1) |
| GSE16186 |
Gene expression profiles of human mesenchymal stem cells stimulated with canonical Wnt signaling |
|
