|
| Status |
Public on Aug 20, 2019 |
| Title |
Salivary supernatant from MPE patient, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Salivary supernatant from MPE patient
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Malignant pleural effusion (MPE)
gender: Male
sample type: Salivary supernatant
|
| Treatment protocol |
All samples were centrifuged at 3000 × g for 15 minutes at 4°C to get rid of the cells. Only the supernatant was transferred into a 2 mL centrifuge tube. Then, to completely remove the cellular components, a further centrifugation of 12000 × g for 10 minutes at 4°C was followed. The supernatant was transferred into a 2 mL centrifuge tube and stored at -80°C until use.
|
| Growth protocol |
Before saliva collection, all patients and healthy controls were required to avoid from eating, drinking or smoking for at least 2 hours. At least 3mL saliva was collected from each subject.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (FlashTag™ Biotin HSR RNA Labeling Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
21.5 μL biotin-labeled sample were hybridized for 16 hr at 48℃ on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol. （Affymetrix® Command Console® User Manual (P/N 702569)）
|
| Description |
Gene expression data from salivary supernatant of MPE patients and healthy controls
MPE3
|
| Data processing |
Use Expression Console software for data summarization, normalization, and quality control.
|
| |
|
| Submission date |
Aug 19, 2019 |
| Last update date |
Aug 21, 2019 |
| Contact name |
Yuan Yang |
| Organization name |
Beijing Chaoyang Hospital, Capital Medical University
|
| Street address |
8 Gongti Nanlu, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100020 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE135947 |
Salivary microRNA expression data from malignant pleural effusion patients and healthy controls |
|
