|
| Status |
Public on Aug 14, 2019 |
| Title |
F4/80+ macrophages 3 days after Kupffer cell depletion F4-80pos_mac_D3_1 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
|
| Treatment protocol |
All Methods listed in Bonnardel et al. Stellate cells, hepatocytes and endothelial cells imprint the Kupffer cell identity on monocytes colonizing the liver macrophage niche . Immunity. 2019.
|
| Growth protocol |
All Methods listed in Bonnardel et al. Stellate cells, hepatocytes and endothelial cells imprint the Kupffer cell identity on monocytes colonizing the liver macrophage niche . Immunity. 2019.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All Methods listed in Bonnardel et al. Stellate cells, hepatocytes and endothelial cells imprint the Kupffer cell identity on monocytes colonizing the liver macrophage niche . Immunity. 2019.
|
| Label |
Biotin
|
| Label protocol |
All Methods listed in Bonnardel et al. Stellate cells, hepatocytes and endothelial cells imprint the Kupffer cell identity on monocytes colonizing the liver macrophage niche . Immunity. 2019.
|
| |
|
| Hybridization protocol |
All Methods listed in Bonnardel et al. Stellate cells, hepatocytes and endothelial cells imprint the Kupffer cell identity on monocytes colonizing the liver macrophage niche . Immunity. 2019.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
F4/80+ macrophages 3 days after Kupffer cell depletion
F4/80+ macrophages 3 days after Kupffer cell depletion
|
| Data processing |
Data were processed using Bioconductor. Normalisation was done by RMA.
All samples passed quality control, and the Robust Multi-array Average (RMA) procedure was used to normalize data within arrays (probeset summarization, background correction and log2-transformation) and between arrays (quantile normalization).
|
| |
|
| Submission date |
Aug 13, 2019 |
| Last update date |
Aug 14, 2019 |
| Contact name |
Liesbet Martens |
| E-mail(s) |
liesbet.martens@irc.vib-ugent.be
|
| Organization name |
VIB-University of Ghent
|
| Department |
VIB Inflammation Research Center
|
| Street address |
Technologiepark 927
|
| City |
Zwijnaarde |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE135788 |
Stellate cells, hepatocytes and endothelial cells imprint the Kupffer cell identity on monocytes colonizing the liver macrophage niche (microarray) |
| GSE135790 |
Stellate cells, hepatocytes and endothelial cells imprint the Kupffer cell identity on monocytes colonizing the liver macrophage niche |
|
