|
| Status |
Public on Mar 16, 2020 |
| Title |
RNAseq_PHF19L_SH4_Replicate3 |
| Sample type |
SRA |
| |
|
| Source name |
Human prostate cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DU145
tissue: Human prostate cancer cell line
|
| Treatment protocol |
None
|
| Growth protocol |
DU145 cells were cultured in Dulbecco´s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Gibco), 1x L-glutamine (Gibco) and 1x Pen/Strep (Gibco).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
(RNA-seq) RNA was extracted with the RNeasy kit (Qiagen) following manufacturer's instruction
Libraries were prepared according to Illumina instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
RNA-seq experiment
|
| Data processing |
RNA-seq analysis: Alignment: Sequence reads were mapped against the hg19 human genome assembly using TopHat (PubMed ID 22383036) with the option ‐g 1.
processed data files format and content: Files *.bdg.gz (BedGraph, genome-wide ChIP-seq/RNA-seq)
Genome_build: hg19
|
| |
|
| Submission date |
Aug 09, 2019 |
| Last update date |
Mar 16, 2020 |
| Contact name |
Enrique Blanco |
| E-mail(s) |
enrique.blanco@crg.eu
|
| Phone |
+34 93 316 01 00
|
| Organization name |
Center for Genomic Regulation (CRG)
|
| Department |
Gene Regulation, Stem Cells and Cancer
|
| Lab |
Epigenetic Events in Cancer (L. Di Croce's lab)
|
| Street address |
Dr. Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE135623 |
PHF19 mediated regulation of proliferation and invasiveness in prostate cancer |
|
| Relations |
| BioSample |
SAMN12542313 |
| SRA |
SRX6686403 |
