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| Status |
Public on Sep 05, 2019 |
| Title |
Pax7Lo |
| Sample type |
SRA |
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| Source name |
Satellite cells
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6:SJL/J
tissue: tibialis anterior muscle
age: 3 month
cell population: Pax7Lo
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| Extracted molecule |
total RNA |
| Extraction protocol |
Cellular suspensions of Pax7Hi, Pax7Mi and Pax7Lo were loaded on a Chromium Controller instrument (10x Genomics) to generate single-cell gel bead-in emulsions (GEMs), respectively. GEM-reverse transcriptions (GEM-RTs) were performed in a Veriti 96-well thermal cycler (Thermo Fisher Scientific). After RT, GEMs were harvested and the cDNAs were amplified and cleaned up with the SPRIselect Reagent Kit (Beckman Coulter)
Indexed sequencing libraries were constructed using the Chromium Single-Cell 30 Library Kit (10x Genomics) for enzymatic fragmentation, end-repair, A-tailing, adaptor ligation, ligation cleanup, sample index PCR, and PCR cleanup.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Cell Ranger version 1.3.1 (10x Genomics) to process raw sequencing data
Seurat suite version 2.0.0 for downstream analysis
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include count value for each Sample
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| Submission date |
Jul 19, 2019 |
| Last update date |
Sep 05, 2019 |
| Contact name |
Yong Zhang |
| E-mail(s) |
yongzhang@ibms.pumc.edu.cn
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| Organization name |
School of Basic Medicine, Peking Union Medical College
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| Street address |
5 Dong Dan San Tiao
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| City |
Beijing |
| ZIP/Postal code |
100005 |
| Country |
China |
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| Platform ID |
GPL24247 |
| Series (2) |
| GSE134253 |
Muscle-secreted G-CSF as a metabolic niche factor ameliorates loss of muscle stem cell in aged mice |
| GSE134540 |
Muscle-secreted G-CSF as a metabolic niche factor ameliorates loss of muscle stem cell in aged mice (scRNA-seq) |
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| Relations |
| BioSample |
SAMN12321588 |
| SRA |
SRX6473684 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM3955374_P4_filtered.csv.gz |
5.0 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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