|
| Status |
Public on Jul 05, 2022 |
| Title |
Diet_Veh_high_1831 CD-HFD Vehicle With HCC and fibrosis |
| Sample type |
RNA |
| |
|
| Source name |
Non-tumor liver tissue from diet-induced model (CD-HFD) of NASH-HCC treated with vehicle, that developed HCC and fibrosis
|
| Organism |
Mus musculus |
| Characteristics |
liver status: Chronic damage
tissue: liver
treatment: Vehicle
|
| Treatment protocol |
As previously published (Malehmir M et al, Nat Med, 2019)
|
| Growth protocol |
As previously published (Malehmir M et al, Nat Med, 2019)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 20 mg fresh-frozen non-tumoral liver tissue with Trizol reagent (Invitrogen) and purified with RNeasy columns (Qiagen, Valencia, CA). RNA sample concentration and quality were assessed by NanoDrop ND-1000 spectrometer (NanoDrop, Wilmington, DE) and bioanalyzer (Agilent, Palo Alto, CA), respectively.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Gene expression from Non-tumor liver tissue from diet-induced model (CD-HFD) of NASH-HCC treated with vehicle, that developed HCC and fibrosis
|
| Data processing |
Raw expression data from microarrays were normalized using the ExpressionFileCreator [RMA with quantile normalization method].
|
| |
|
| Submission date |
Jul 08, 2019 |
| Last update date |
Jul 05, 2022 |
| Contact name |
Sara Torrecilla |
| Organization name |
IDIBAPS
|
| Lab |
Liver Cancer Translational Research Laboratory
|
| Street address |
Rossello Street, 149
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE133969 |
An Immune Gene Expression Signature Associated With Development of Human Hepatocellular Carcinoma Identifies Mice That Respond to Chemopreventive Agents II |
|
