|
| Status |
Public on May 28, 2009 |
| Title |
UPN44S_T-cell |
| Sample type |
genomic |
| |
|
| Source name |
T-cell fraction
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: T-cell fraction
disease state: MDS
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
QiaAmp purification kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated DNA was prepared according to the standard Affymetrix protocol from two times 250 ng genomic DNA (Genome-Wide Human SNP Nsp/Sty 6.0 User Guide manual 702504 Rev.1, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridizations were performed according to the standard Affymetrix protocol from two times 250 ng genomic DNA (Genome-Wide Human SNP Nsp/Sty 6.0 User Guide manual 702504 Rev.1, Affymetrix) using an Affymetrix hybridisation oven 640 and an Affymetrix Fluidic station 450.
|
| Scan protocol |
Scanning performed according to the standard Affymetrix protocol from two times 250 ng genomic DNA ((Genome-Wide Human SNP Nsp/Sty 6.0 User Guide manual 702504 Rev.1, Affymetrix) using an Affymetrix scanner 3000.
|
| Description |
FACs-sorted T-cell fraction
|
| Data processing |
CNAG 2.0 and Affymetrix Genotypic Console 2.1
|
| |
|
| Submission date |
Apr 15, 2009 |
| Last update date |
May 28, 2009 |
| Contact name |
Roland P. Kuiper |
| E-mail(s) |
r.kuiper@antrg.umcn.nl, e.verwiel@antrg.umcn.nl
|
| Phone |
+31243610868
|
| Fax |
+31243668752
|
| Organization name |
Radboud University Nijmegen Medical Centre
|
| Department |
Human Genetics
|
| Street address |
Geert Grooteplein 10
|
| City |
Nijmegen |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE15688 |
Acquired mutations in TET2 are common in myelodysplastic syndromes |
|
