|
| Status |
Public on Apr 15, 2009 |
| Title |
Genomic DNA isolated from C57BL6/J mice at E17.5, chr11 to chrY, biol rep2 |
| Sample type |
genomic |
| |
|
| Source name |
Genomic DNA isolated from C57BL6/J mice at E17.5, chr11 to chrY, biol rep2
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
age: E17.5
tissue: liver
|
| Growth protocol |
C57BL6/J mice (Jackson Laboratories, Bar Harbor, ME) were used. For collection of fetal liver samples, timing of coitus was determined by observation of vaginal plugs; the morning a plug was observed was considered 0.5 days post coitum. At 17.5 days post coitum (E17.5), pregnant females were killed by CO2 asphyxiation, and fetal liver collected. P21 liver was collected directly after removing mice from their dams in the morning.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using a standard phenol–chloroform method
|
| Label |
Cy3
|
| Label protocol |
Followed Agilent protocol
|
| |
|
| Hybridization protocol |
Followed Agilent protocol
|
| Scan protocol |
Scanned on an Agilent Technologies Scanner G2505B US23502362
Images were quantified using Agilent Feature Extraction Software (version 9.1.1.1).
|
| Description |
Methylated DNA enriched by the MCAM method. Biological replicate 2 of 2.
|
| Data processing |
Agilent Feature Extraction Software (9.1.1.1) was used for background subtraction and normalization, default normalization procedure.
|
| |
|
| Submission date |
Apr 14, 2009 |
| Last update date |
Apr 14, 2009 |
| Contact name |
Jiexin Zhang |
| E-mail(s) |
jiexinzhang@mdanderson.org
|
| Organization name |
UT MD Anderson Cancer Center
|
| Department |
Bioinformatics & Computational Biology
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6612 |
| Series (1) |
| GSE15634 |
Methylation changes occurring in early postnatal liver development |
|
