|
| Status |
Public on Aug 05, 2020 |
| Title |
Sham3 |
| Sample type |
RNA |
| |
|
| Source name |
ob/ob sham islets, biological replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: pancreatic islets
strain: C57Bl/6J
gender: male
|
| Treatment protocol |
Pancreatic islets were isolated from lean (WT) mice or from ob/ob mice, 28 days after sham or EGA surgery.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the miRNeasy kit (Qiagen), according to manufacturer guidelines.
|
| Label |
Cy3
|
| Label protocol |
Total RNA from each sample was amplified and transcribed into fluorescent Cyanine-3 (cy3) cRNA using the manufacturer’s Agilent’s Quick Amp Labeling protocol (version 5.7, Agilent Technologies).
|
| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized onto the Whole Mouse Genome Oligo Microarray (4 x 44K, Agilent Technologies) based on the manufacturer’s standard protocols.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent Scanner G2505C.
|
| Description |
Gene expression 28 days after sham surgery
|
| Data processing |
Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the GeneSpring GX v12.1 software (Agilent Technologies). After quantile normalization of the raw data, genes that at least 3 out of 6 samples have flags in Detected (“All Targets Value”) were chosen for further data analysis.
|
| |
|
| Submission date |
Jul 01, 2019 |
| Last update date |
Aug 05, 2020 |
| Contact name |
Claudiane Guay |
| E-mail(s) |
claudiane.guay@unil.ch
|
| Phone |
+41216925281
|
| Organization name |
University of Lausanne
|
| Department |
DNF
|
| Street address |
Du Bugnon 9
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1005 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL11202 |
| Series (2) |
| GSE133577 |
Effects of entero-gastro-anastomosis (EGA) surgery in ob/ob mice on pancreatic islet gene expression |
| GSE133852 |
ob/ob mice on pancreatic islet |
|
