|
| Status |
Public on Jul 03, 2009 |
| Title |
468GFP biorep2 |
| Sample type |
genomic |
| |
|
| Source name |
Parental Cell line 468GFP, second biological replicate
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 468GFP
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Cells were grown out to three passages in alpha-MEM containing 10% FBS in 5% CO2 to 70 % confluency
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Sigma GeneElute kit using the Manufacturer's protocol,
|
| Label |
Biotin/SAPE
|
| Label protocol |
Genomic DNA was labeled was labeled as per Affymetrix standard protocol for Human SNP6.0 arrays
|
| |
|
| Hybridization protocol |
Genomic DNA was hybridized as per standard affymetrix protocols for Human SNP6.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Human SNP 6.0 array data (affymetrix) from Parental Cell line 468GFP, second biological replicate
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method. .CEL files thus produced were imported into Partek Genomics Suite software and background subtracted using the RMA algorithm.
|
| |
|
| Submission date |
Apr 09, 2009 |
| Last update date |
Jul 02, 2009 |
| Contact name |
Ann Chambers |
| E-mail(s) |
joseph.andrews@lhsc.on.ca
|
| Phone |
(519) 685-8430
|
| Organization name |
London Regional Cancer program
|
| Department |
Victoria research labs
|
| Street address |
790 Commissioner's Road E
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 4L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6801 |
| Series (2) |
| GSE14867 |
SNP/CNV analysis in a breast cancer metastasis model |
| GSE15619 |
Epigenetic signature of breast cancer and its association with gene expression and copy number |
|
