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Sample GSM385707 Query DataSets for GSM385707
Status Public on Mar 26, 2009
Title Deep-sowing 10d treatment IAA, biological rep1
Sample type RNA
 
Source name Mesocotyl, IAA
Organism Zea mays
Characteristics genotype: Maize inbred line 3681-4
age: 10d after sowing
tissue: mesocotyls
Extracted molecule total RNA
Extraction protocol The total RNA was extracted by the TRIzol method (Invitrogen, USA). Mesocotyls were ground in liquid nitrogen, and 50-100 mg of frozen tissue was transferred into a tube with 1 ml TRIzol. Next, RNA was isolated, dried and dissolved in 50 µl DEPC-H2O. RNA quality and concentration were determined by 1.0% agarose gel electrophoresis and spectrophotometry.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45℃ on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from maize mesocotyl
Data processing The data were analyzed with GCOS software (Microarray Suite version 5.0, MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Mar 24, 2009
Last update date Mar 25, 2009
Contact name Guoying Wang
E-mail(s) gywang@caas.net.cn
Phone 86-10-62162927
Organization name Chinese Academy of Agricultural Sciences
Department Institute of Crop Sciences
Lab Maize Molecular Biology
Street address Zhongguancun Nandajie 12
City Beijing
ZIP/Postal code 100081
Country China
 
Platform ID GPL4032
Series (1)
GSE15371 Auxin-induced gene expression in mesocotyl elongation of maize inbred line 3681-4 tolerant to deep-sowing

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 97.869 P 0.00110197
AFFX-BioB-M_at 96.704 P 0.000296381
AFFX-BioB-3_at 97.5073 P 0.000340305
AFFX-BioC-5_at 256.331 P 8.14279e-05
AFFX-BioC-3_at 297.051 P 4.42873e-05
AFFX-BioDn-5_at 420.215 P 4.42873e-05
AFFX-BioDn-3_at 1510.19 P 8.14279e-05
AFFX-CreX-5_at 3744.68 P 5.16732e-05
AFFX-CreX-3_at 4988.25 P 4.42873e-05
AFFX-DapX-5_at 842.135 P 4.42873e-05
AFFX-DapX-M_at 1520.53 P 5.16732e-05
AFFX-DapX-3_at 2364.5 P 4.42873e-05
AFFX-LysX-5_at 122.444 P 4.42873e-05
AFFX-LysX-M_at 154.414 P 0.00010954
AFFX-LysX-3_at 619.8 P 4.42873e-05
AFFX-PheX-5_at 261.705 P 4.42246e-05
AFFX-PheX-M_at 275.877 P 5.16732e-05
AFFX-PheX-3_at 320.87 P 4.42873e-05
AFFX-ThrX-5_at 236.37 P 4.42873e-05
AFFX-ThrX-M_at 461.295 P 4.42873e-05

Total number of rows: 17734

Table truncated, full table size 654 Kbytes.




Supplementary file Size Download File type/resource
GSM385707.CEL.gz 2.2 Mb (ftp)(http) CEL
GSM385707.CHP.gz 96.6 Kb (ftp)(http) CHP
Processed data provided as supplementary file
Processed data included within Sample table

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