|
| Status |
Public on Jun 19, 2019 |
| Title |
KO2 |
| Sample type |
SRA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
genotype: NSUN2-/-
tissue: liver
molecule subtype: mitochondrial enriched RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mouse liver tissue lysate was enriched for mitochondria. RNA was treated with Ribo Zero Gold and DNase. Bisulfite treatment (Epigentek, three cycles of 90°C for 5 min and 60°C for 1h)
Illumina TruSeq small RNA
|
| |
|
| Library strategy |
Bisulfite-Seq |
| Library source |
transcriptomic |
| Library selection |
RANDOM |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Quality and 3' end adapter trimming with Trim_Galore!
Reads longer than 20nt were aligned to GRCh38 with Bismark
Genome_build: GRCh38
|
| |
|
| Submission date |
May 31, 2019 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Lindsey Van Haute |
| Organization name |
University of Cambridge
|
| Department |
MRC Mitochondrial Biology Unit
|
| Street address |
Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE132020 |
NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs |
|
| Relations |
| BioSample |
SAMN11898188 |
| SRA |
SRX5938165 |
