|
| Status |
Public on May 13, 2009 |
| Title |
Root_Control3 |
| Sample type |
RNA |
| |
|
| Source name |
Barley roots grown in control conditions
|
| Organism |
Hordeum vulgare |
| Characteristics |
tissue: root
agent: control
|
| Growth protocol |
Barley (Hordeum vulgare L. cv. Graphic) seeds were carefully chosen with similar weight, shape and size and sown in 1 L pots containing sand in a glasshouse for 15 days (Experimental Fields of the University of Barcelona) and irrigated with a half strength Hoagland solution containing either 0 µM (control), 500 µM or 1000 µM of HgCl2. Daily means of temperature, relative humidity and maximum irradiance in the glasshouse throughout the experiment were 16ºC, 45% and 378 Wm-2, respectively. For root and shoot dry weight, 5 plantlets were sampled, immediately frozen in liquid nitrogen, lyophylized and dry weights were recorded and shoot to root ratio calculated. Additional three plantlets per treatment (only controls and 1000 μM HgCl2) were sampled and the entire roots were separated from leaves, washed with de-ionized water and immediately frozen in liquid N and kept at -80ºC until use for RNA extractions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent and Rneasy Quiagen
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix procedures.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures.
|
| Scan protocol |
Standard Affymetrix procedures.
|
| Description |
All sample extraction and micro chip manipulation were carried out at PROGENIKA, in SPAIN (Bilbao)
|
| Data processing |
Normalization was carried out in 2 steps: using GeneSpring v7.1 (Agilent) and previously scaled with GCOS.
|
| |
|
| Submission date |
Mar 19, 2009 |
| Last update date |
May 12, 2009 |
| Contact name |
Marta Silva Lopes |
| Fax |
525558047558
|
| Organization name |
cimmyt
|
| Department |
wheat physiology
|
| Street address |
km 45, carretera Mexico-Veracruz, El Batan, Texcoco
|
| City |
Texcoco |
| State/province |
Mexico |
| ZIP/Postal code |
56130 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL1340 |
| Series (1) |
| GSE15295 |
Mercury toxicity in barley roots |
|
