|
| Status |
Public on Aug 08, 2019 |
| Title |
KO_b_8h |
| Sample type |
RNA |
| |
|
| Source name |
Esrrb KO ESCs, treated 8hrs and subsequently 2hrs with Mek inhibitor
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Esrrb Knockout Expressing Raf-ErT2
cell type: ESCs
strain: 129SV
protocol: Empty Vector Control
|
| Treatment protocol |
Cells were left untreated (0h), or treated with 250nM 4OHT (2h, 8h), or treated with 4OHT for 8hrs followed by a subsequent 2h Mek inhibitor treatment (8_2h).
|
| Growth protocol |
mESCs were grown in serum containing medium, supplemented with 1x LIF (made in house) and 2uM Chir99021. 24hrs prior to treatment, media was changed to serum containing media supplemented with 1x LIF and 250nM PD173074.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was collected using the RNeasy Mini Kit (Qiagen). Genomic DNA was eliminated by DNAse treatment (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
RNA was amplified into cRNA and 100ng of total RNA was labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies- 5190-2305).
|
| |
|
| Hybridization protocol |
Samples were then hybridized to the G4852A SurePrint G3 Mouse GE 8x60K Microarray slides (60000 probes, Agilent Technologies).
|
| Scan protocol |
Images were scanned using the high sensitivity protocol (AgilentG3_HiSen_GX_1color) on a SureScan microarray scanner (Agilent Technologies) and probe intensities were obtained by taking the gProcessedSignal from the output of Agilent feature extraction software using default settings.
|
| Description |
Gene expression data from Esrrb KO ESCs, treated 8hrs and subsequently 2hrs with Mek inhibitor
|
| Data processing |
gProcessed signal
|
| |
|
| Submission date |
May 21, 2019 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Josh M Brickman |
| E-mail(s) |
joshua.brickman@sund.ku.dk
|
| Organization name |
Copenhagen University
|
| Department |
reNEW
|
| Lab |
Brickman
|
| Street address |
3B Blegdamsvej
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL21810 |
| Series (2) |
| GSE131546 |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation [gene expression] |
| GSE132444 |
Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation |
|
