|
| Status |
Public on Mar 01, 2010 |
| Title |
human embryonic stem cells FES29 p37 |
| Sample type |
RNA |
| |
|
| Source name |
human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human embryonic stem cell
cell line: FES29 p37
gender: male
|
| Treatment protocol |
None
|
| Growth protocol |
standard for human embryonic stem cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cell pellets using Rneasy Kit (Qiagen). To eliminate genomic DNA from the samples Dnase I (Qiagen) digestion in column was included.
|
| Label |
biotin
|
| Label protocol |
accordingly to the manufacturer's instructions (Affymetrix)
|
| |
|
| Hybridization protocol |
accordingly to the manufacturer's instructions (Affymetrix)
|
| Scan protocol |
accordingly to the manufacturer's instructions (Affymetrix)
|
| Description |
Hybridized in Finnish DNA microarray Centre
|
| Data processing |
Probe values were directly linked to Ensembl genes (build 49) using alternative CDF-files provided by brainarray (http://brainarray.mbni.med.umich.edu/), version 11. R package aroma.affymetrix was used in analyzing the gene values of the expression and RMA for pre-processing the Exon array values.
|
| |
|
| Submission date |
Mar 04, 2009 |
| Last update date |
Jan 29, 2010 |
| Contact name |
Elisa Maarit Närvä |
| E-mail(s) |
elisa.narva@btk.fi
|
| Organization name |
University of Turku
|
| Department |
Turku Centre for Biotechnology
|
| Lab |
Riitta Lahesmaa
|
| Street address |
Tykistönkatu 6
|
| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL8238 |
| Series (2) |
| GSE15095 |
Exon arrays for human embryonic stem cells |
| GSE15097 |
High resolution genome wide DNA analysis on a large panel of Human Embryonic stem cell lines |
|
