|
| Status |
Public on May 16, 2019 |
| Title |
TAC8w33 polysome RNA |
| Sample type |
SRA |
| |
|
| Source name |
heart tissue
|
| Organism |
Mus musculus |
| Characteristics |
experimental condition: TAC (transverse aortic constriction) treated (TAC) mouse (ID 33) after 8 weeks (8w)
merip antibody: none
sample type: Polysomal RNAseq
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, treated with DNAse and cleaned up with Zymoresearch RNA clean-up and concentrator kit.
Libraries were constructed according to Illumina's protocol for TrueSeq stranded RNA library prep kit
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.4
Sequencing reads were alligned to mm10 and hg38 using STAR tools
Low quality reads were filtered out and onlys readz >30 qc were rpcessed further
MeRIP peaks were identified using MeTPeak package
Genome_build: mm10, hg38
Supplementary_files_format_and_content: Gene count files are provided as processed files, with read numbers detected and summed per gene for given sample.
|
| |
|
| Submission date |
May 15, 2019 |
| Last update date |
May 16, 2019 |
| Contact name |
Gaurav Jain |
| E-mail(s) |
gauravj49@gmail.com
|
| Phone |
+49-551-39-61194
|
| Organization name |
The German Center for Neurodegenerative Diseases (German: Deutsches Zentrum für Neurodegenerative Erkrankungen DZNE)
|
| Street address |
Von-Siebold-Str. 3a, Office no: 2.212
|
| City |
Göttingen |
| State/province |
Lower Saxony |
| ZIP/Postal code |
37075 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE131296 |
Changes in m6A RNA methylation contribute to heart failure progression by modulating translation |
|
| Relations |
| BioSample |
SAMN11655434 |
| SRA |
SRX5839972 |
